panc1 human pdac cells male  (ATCC)

Journal: Cell reports

Article Title: Reprogramming of nucleotide metabolism by interferon confers dependence on the replication stress response pathway in pancreatic cancer cells

doi: 10.1016/j.celrep.2021.110236

Figure Lengend Snippet: (A) Principal-component analysis (PCA) of fold changes in 485 metabolites measured by LC-MS in SUIT2, YAPC, and PANC1 cells treated ±100 U/mL IFNβ for 24 h (n = 6). Numbers and percentages of metabolites significantly altered by IFNβ treatment are indicated (FDR ≤ 10%). (B) Metabolite set enrichment analysis (MSEA) of significantly altered metabolites in each cell line. (C) Summary of IFN-induced alterations in nucleotide and NAD+/NADH metabolism in SUIT2 cells. (D) Relative levels of indicated metabolites in PDAC cell lines treated ±100 U/mL IFNβ for 24 h (mean ± SD, n = 6, unpaired t test). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Human PANC1 (male) cells , ATCC , Cat#CRL1469; RRID:CVCL_0480.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: Cell reports

Article Title: Reprogramming of nucleotide metabolism by interferon confers dependence on the replication stress response pathway in pancreatic cancer cells

doi: 10.1016/j.celrep.2021.110236

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Human PANC1 (male) cells , ATCC , Cat#CRL1469; RRID:CVCL_0480.

Techniques: Virus, Microarray, Recombinant, Lysis, Protease Inhibitor, Membrane, Saline, Autoradiography, Plasmid Preparation, Control, BIA-KA, Reverse Transcription, Enzyme-linked Immunosorbent Assay, Expressing, Software, Mass Spectrometry, Targeted Proteomics, Real-time Polymerase Chain Reaction, Live Cell Imaging